phospho irf 3 ser396 4d4g rabbit mab (Cell Signaling Technology Inc)
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phospho irf 3 ser396 4d4g rabbit mab
Phospho Irf 3 Ser396 4d4g Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+irf+3+ser396+4d4g+rabbit+mab/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pm41864138-50-0-32
Average 96 stars, based on 1416 article reviews
Phospho Irf 3 Ser396 4d4g Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+irf+3+ser396+4d4g+rabbit+mab/Phospho-IRF-3+(Ser396)+Rabbit+mAb/pm41864138-50-0-32
Average 96 stars, based on 1416 article reviews
phospho irf 3 ser396 4d4g rabbit mab - by Bioz Stars,
2026-09
96/100 stars
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Purification:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Bicinchoninic Acid Protein Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Protease Inhibitor:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Ethidium Homodimer Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Modification:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Recombinant:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Clinical Proteomics:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), CCK-8 Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Enzyme-linked Immunosorbent Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Blocking Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Immunopeptidomics:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Cell Isolation:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Staining:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), ROS Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), cDNA Synthesis:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), SYBR Green Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Plasmid Preparation:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Software:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Microscopy:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Transmission Assay:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), Gene Expression:Article Title: Vinorelbine causes a neuropathic pain-like state in mice via STING and MNK1 signaling associated with type I interferon induction Article Snippet: Article Title: Bimetallic Nanoreactor Activates cGAS‐STING Pathway via mtDNA Release for Cancer Metalloimmunotherapy Article Snippet: Materials: The following materials were used without further purification: hydrogen peroxide (H2O2, 34.5 wt.%) and dimethyl sulfoxide (DMSO) (SAMCHUN); PEG 200 (DAJUNG); calcium chloride dihydrate (CaCl2•2H2O), ammonium hydroxide solution (NH3•H2O, 28%– 30% wt.%), potassium permanganate (KMnO4), oleic acid, branched PEI-g-PEG, curcumin (CUR), 3,3′,5,5′-tetramethylbenzidine (TMB), fluorescein isothiocyanate (FITC), rhodamine B isothiocyanate (RITC), penicillin–streptomycin, β-mercaptoethanol, formalin solution (neutral buffered, 10%), fetal bovine serum (FBS), ReadyShield phosphatase inhibitor cocktail, and [Ru(dpp)3]Cl2 (Sigma–Aldrich); Amplex Red hydrogen peroxide/peroxidase assay kit, cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), BCA protein assay kit, RIPA buffer, protease inhibitor cocktail, calcein-AM, ethidium homodimer-1 (EthD-1), Fluo4-AM cell-permeant, 4′,6-diamidino-2-phenylindole (DAPI), QuantiT PicoGreen dsDNA reagents, MitoTracker Red CMXRos, and STING polyclonal antibody (Invitrogen); Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI 1640) medium, non-essential amino acids, geneticin, and HEPES (Gibco); recombinant murine granulocyte-macrophage colony-stimulating factor (GM– CSF) (PeproTech); cell counting kit-8 (CCK-8) (Dojindo); InVivoMAB antimouse PD-1 (CD279) (BioX cell); rabbit anti-mouse TFAM polyclonal antibody (MyBioSource); anti-8-hydroxy-2′-eoxyguanosine (8-OHdG) antibody (Abcam); mouse IL-6, TNF-α, IFN-β, and IFN-γ DuoSet ELISA kits (R&D Systems, USA); FcR blocking reagent (Miltenyi Biotec); and CD11c-APC, CD86-eFlour 450, CD80-eFlour 450, MHC-II-FITC, SIINFEKLMHC-I-PE-cyanine 7, F4/80-PE, CD3-FITC, CD4-PE-cyanine 7, CD8-APC, CD44-eFlour 450, Foxp3-APC, and CD62L-PE (eBioscience); 2′3′-cGAMP (cGAMP, InvivoGen); phospho-NF-κB p65 (Ser536) (93H1) rabbit mAb (anti-p-p65), phospho-IRF-3 (Ser396) (4D4G) rabbit mAb (anti-p-IRF-3), NF-κB p65 (D14E12) XP rabbit mAb (anti-p65), IRF-3 (D83B9) rabbit mAb (anti-IRF-3), phospho-STING (Ser365) (D8F4W) rabbit mAb (antip-STING), |